[Hplusroadmap] Fwd: Poor man's spectrophotometer for proteins

[Bryan Bishop]

----------  Forwarded Message  ----------

Subject: Poor man's spectrophotometer for proteins
Date: Thursday 29 May 2008
From: Julie E Norville <norville at mit.edu>
To: diybio at googlegroups.com


If you do affinity chromatography (or any other type of chromatography) 
for
proteins, one simple way to check and see if the protein is there is to 
tap the
tube and see if bubbles appear.  If bubbles readily appear that 
indicates the
presence of a protein at high concentration.
This is what they used to do to find potentially good fractions in labs 
that
didn't want to buy a spectrophotometer.  You will not know from this 
whether
the protein is pure.

Polyprep columns from Biorad are pretty cheap.

Affinity resins are not very reasonably priced this point of time.


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