[Hplusroadmap] [Synthetic Biology] Use a laser-inducable writozyme to make a plasmid, which then inserts genes

[Bryan Bishop]

1) Writozyme makes a plasmid from scratch.
1.1) Laser denatures or renatures etc. the writozyme for a certain nt
*** hack the mechanism of DNA polymerase III? (long live Kornberg)
1.2) The nt attaches to the writozyme
*** Random brownian diffusion here. Nothing special.
1.3) The writozyme applies some energy to connect the nt to the strand
*** Actually, the writozyme wouldn't do this - other diffused enzymes.
1.4) Ideally, pop the stack with a pingback for confirmation.
1.5) When done, connect the ends of the DNA strand together.
*** Writozyme is the block in the mean time.

2) Plasmid transfers the gene into the cell.

3) Done. Also, this ensures a one-to-one correspondence, since a 
population of the same plasmids should not be able to operate more than 
once on a single cell, right? You have many writozymes doing the same 
thing at once, but only one plasmid per cell, so this is how you get 
your determinism.

Thoughts?

- Bryan
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Bryan Bishop
http://heybryan.org/