[Hplusroadmap] Question about current DNA sequencing technology?

[Bryan Bishop]

On Tuesday 04 March 2008, Dan Bolser wrote:
> Sorry for the dumb question, but what is the current 'state of the
> art' DNA sequencing technology? How fast? How accurate?

I saw some recent emails on this topic, just went back to forward them.

> What is 'next generation' sequencing (in real terms?).

http://heybryan.org/mediawiki/index.php/Nanopore

Or see ZS Genetics, they plan to release STM DNA sequencing later this 
year. I have some notes up on the wiki for this as well, it's something 
that I'd like to try out. 

http://heybryan.org/mediawiki/index.php/DNA_sequencer#Scanning_Tunneling_Microscopy_.28STM.29_DNA_sequencing

STM machines can be built at home for ~$100 and get nearly 10 nm 
resolution. Without super-powerful vacuums.

> Any info on the wiki?

Somewhat. I actually lack knowledge of the fluorescent methods that are 
mentioned in the forwarded emails, so if you can help me out there, 
that'd be great.

> It would be really great to see a time line of DNA sequencing
> technology ... any links?

This might be the cloest thing:
http://en.wikipedia.org/wiki/Timeline_of_biology_and_organic_chemistry

Also, surely there must be a review article out there in the databases 
somewhere. Perhaps PubMed?

- Bryan
________________________________________
Bryan Bishop
http://heybryan.org/