[Hplusroadmap] Fwd: Re: Bio Heath Kit

[William Heath]

Check this cool heathkit type cell thing I found:


http://www.synberc.org/news.html


On Tue, Apr 8, 2008 at 3:04 PM, Bryan Bishop <kanzure at gmail.com> wrote:

>
> ----------  Forwarded Message  ----------
>
> Subject: Re: Bio Heath Kit
> Date: Tuesday 08 April 2008
> From: Kim <Kim.deMora at gmail.com>
> To: DIYbio <diybio at googlegroups.com>
>
>
> Hey guys,
>
>  Using fluorescence can be difficult.  If you are working with yeast,
> the expression levels can be low enough that you need a fluorescence
> microscope to see whats going on.  Maybe that would be an interesting
> project:  How to modify a cheap microscope to see fluorescence.
> Failing that, RFP expression in E. coli is visible to the naked eye if
> you are using a strong enough promoter.  Another reporter would be to
> use the Lac operon to produce a drop in pH in the growth medium (for
> liquid cultures).  This method only needs a relatively inexpensive pH
> meter or even disposable litmus paper strips, although it would be
> difficult to get more than a yes/no answer without being clever.
>
> Something that is very important to any kind of microbiology work is
> the ability to keep everything sterile.  Ideally this is done using an
> autoclave, but for sterilizing petri dishes/anything metal, you could
> feasibly use 70% ethanol and a flame.
>
>  You also need the ability to select which cells have the DNA that
> you want in them and which don't.  This is usually done using drug
> resistance plasmids in E. coli, but beta-gal selection might be a more
> feasible and cheap option for do-it-yourself work.  Cheers,
>
> Kim
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