[Hplusroadmap] Fwd: Re: Garagista Protocols and Equipment

[Mackenzie Cowell]

Sorry, $10!  It's an innovative convective PCR thermocycler.

By applying a static temperature gradient across an appropriately designed
reactor geometry (e.g., a cylindrical cavity or closed loop), a continuous
circulatory flow can be initiated that will repeatedly transport PCR
reagents through temperature zones associated with each stage of the
reaction.

See:
http://www.scribd.com/doc/1827003/Pocketsized-Convective-PCR-Thermocycler
Also: http://npg.nature.com/nmeth/journal/v3/n1/pdf/nmeth0106-8b.pdf

mac

On Tue, Apr 8, 2008 at 6:07 PM, Bryan Bishop <kanzure at gmail.com> wrote:

>
> On Tuesday 08 April 2008, Mackenzie Cowell wrote:
> > My personal interest is in developing and testing cheap, off the
> > shelf experimental hardware like the $15 pcr machine, or a centrifuge
>
> What's the $15 PCR machine?
>
> > based on a wheelchair motor or electric drill, or a laminar-flow hood
>
> In the biohacking zip there's the suggestion of making it out of an old
> bicycle. Just peddle away. But an electric drill might be an ever
> better idea, even I have one of those laying around here somewhere.
>
> > built from a large tupperware crate and a desk fan, etc.  I think we
> > are going to need cheaper homebrew substitutes for a lot of the
> > typical lab equipment before we can begin doing many of the
> > interesting wetlab experiments.
>
> That's definitely an issue. Once we do get cheap equipment ideas
> bouncing around, I suggest we focus on setting up some cell cultures in
> our geographic regions for distribution to boostrap various projects
> and experiments, as long as we can feed our cell cultures it "pays" for
> itself.
>
> - Bryan
> ________________________________________
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