[Hplusroadmap] Fwd: Re: Garagista Protocols and Equipment

[Mackenzie Cowell]

Hi Lucy,
Putting together an initial list of practical experiments is a good idea.

My personal interest is in developing and testing cheap, off the shelf
experimental hardware like the $15 pcr machine, or a centrifuge based on a
wheelchair motor or electric drill, or a laminar-flow hood built from a
large tupperware crate and a desk fan, etc.  I think we are going to need
cheaper homebrew substitutes for a lot of the typical lab equipment before
we can begin doing many of the interesting wetlab experiments.

The first diybio meeting is on May 1.  I'm sure we'll discuss "low-hanging"
experiments at the meeting, and I hope that the list will be able to
brainstorm some ideas to fuel the discussion in the meantime.

For instance, I believe Jason Kelly was doing some interesting work with a
particular moss (P. patens) that was rather simple to grow and manipulate
genetically in a kitchen.  Maybe he could share some of his experiences or
ideas for potential experiments.

Happy Hacking,
Mac

On Tue, Apr 8, 2008 at 12:26 AM, <mantese at msn.com> wrote:

>  Mac,
> Hi. Thanks a lot for your last message. Apologies if you receive this
> message twice.
>
> Do you have an idea of what types of garage-type experiments you're
> thinking
> of doing?
>
> I'm running into a roadblock in trying to decide what equipment i need.
> Isn't the first step to decide what experiment to tackle? After that's
> decided
> then it'd be easier to get the necessary equipment.
>
> Maybe we can decide on some "garage-type" experiments and then form a
> DNA-hacker competition around those...similar to the iGEMS format?
>
> BTW, if anyone is in the Calif Bay Area and would like to collaborate on
> some wet-lab experiments, please let me know.
>
> Lucy
>
>
>
> > ---------- Forwarded Message ----------
> >
> > Subject: Re: Garagista Protocols and Equipment
> > Date: Sunday 06 April 2008
> > From: Bryan Bishop <kanzure at gmail.com>
> > To: "Mackenzie Cowell" <macowell at gmail.com>
> >
> > On Sunday 06 April 2008, "Mackenzie Cowell" <macowell at gmail.com> wrote:
> > > Howdy Brian,
> > > Thanks for posting our intro DIYBIO email.
> >
> > You bet.
> >
> > > We need to start aggregating a list of wetlab tools and techniques
> > > that might be practical to use in a garage or kitchen - stuff like
> > > the $15 PCR machine and substituting jello for agar, or building a
> > > centrifuge from an old dryer, etc. Have you already started a list
> > > like this on your site somewhere?
> >
> > Absolutely. There's a few ways to get this list:
> >
> > http://biohack.sf.net/ <- in the zip file
> >
> > I also have lists on my websites.
> > http://heybryan.org/mediawiki/
> >
> > Check out the bottom of that page for some more links. I also have tens
> > of thousands of bookmarks on my local machine, some of which I'll share
> > now that might be related to DIY biology. You may want to organize a
> > hackathon or something for a group of people to go through this huge
> > list sometime.
> >
> > General biotech
> >
> > http://heybryan.org/school/Biology/notes/01-24-07,
> > %20Deoxyribonucleic%20acid.html
> >
> http://news.thomasnet.com/IMT/archives/2006/07/amateur_innovation_basement_biotechnology_diy_engineering.html?t=archive
> > http://www.coolest-gadgets.com/20070613/analyze-your-dna-from-home/
> > http://web.archive.org/web/20070420204234/http://www.dnahack.com/
> > http://www.pnl.gov/news/release.asp?id=171
> > http://www.eubios.info/SG/SG17.htm
> > http://www.nslij-genetics.org/microarray/
>
>
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>
>
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