[Hplusroadmap] Fwd: Re: Garagista Protocols and Equipment

[Bryan Bishop]

On Monday 07 April 2008, mantese at msn.com wrote:
> Mac,Hi. Thanks a lot for your last message. Apologies if you receive
> this message twice. Do you have an idea of what types of garage-type
> experiments you're thinking of doing? I'm running into a roadblock in
> trying to decide what equipment i need. Isn't the first step to
> decide what experiment to tackle? After that's decided then it'd be
> easier to get the necessary equipment. Maybe we can decide on some
> "garage-type" experiments and then form aDNA-hacker competition
> around those...similar to the iGEMS format?  BTW, if anyone is in the
> Calif Bay Area and would like to collaborate onsome wet-lab
> experiments, please let me know.

First order of business would be some simple experiments mimicing common 
protocols. Basically, you need to go identify protocols and figure out 
what they could be used for, then come up with a neat way to show to 
yourself [or others] that you know what they mean and can be used for. 
Then you can move on to some other ideas, like trying to build a DNA 
syntehsizer if you want to. Until then, running a diverse bacteria farm 
might be enough fun. Once you have a stable supply, can you try 
directed evolution experiments? And so on.

- Bryan
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