DNA microarraying on compact disc surface
From Biohack
Microimmunoanalysis on Standard Compact Discs
To Determine Low Abundant Compounds Sergi Morais,† Javier Carrascosa,‡ David Mira,‡ Rosa Puchades,† and Angel Maquieira*,†
DepartamentodeQuı´mica,InstitutodeQuı ´micaMolecularAplicada,andCentrodeTecnologı´aNanofoto´ nicadeValencia, UniversidadPolite´ cnicadeValencia,CaminodeVeras/n,46071Valencia,Spain
High-density competitive indirect microimmunoassays were performed in both sides of compact discs by direct absorption of immunoreagents on polycarbonate surface, using gold- or enzyme-labeled immunoglobulins as tracers for displaying the immunoreaction. The operational principle is based on the use of a low-reflectivity compact disc as analytical platform that allows the reflection/transmission (30/70%) of the CD reader laser beam (λ 780 nm). The reflected light is used to scan the disc track keeping it in movement. The transmitted light is detected by a planar photodiode integrated on the CD drive. The variation of the optical transmission of the light caused by the immunoreaction products is related to the sample concentration. As a proof of concept, low abundant compounds, commonly used as pesticides, were detected in a 60-min total assay time, with a limit of detection ranging from 0.02 to 0.62 µg/L for 2,4,5-TP, chlorpyriphos, and metolachlor. The obtained results show the enormous prospective of compact discs in combination with CD players for multiresidue and drug discovery applications.
DNA microarraying on compact disc surfaces. Application to the analysis of single nucleotide polymorphisms in Plum pox virus Tools and Resources
Sergi Morais, Raquel Marco-Molés, Rosa Puchades* and Ángel Maquieira* Departamento de Química, Universidad Politécnica de Valencia, Camino de Vera s/n, Valencia, Spain. E-mail: rpuchades@qim.upv.es; amaquieira@qim.upv.es
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Received (in Cambridge, UK) 4th January 2006, Accepted 5th April 2006
First published on the web 2nd May 2006
The potential of using compact discs as high throughput screening platforms for DNA microarraying is discussed and applied to discriminate genetic variations of Plum pox virus.
